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**News Article: European Union Approves Methods for Testing Feed Additive NITTEN DFAIII**
The European Union Reference Laboratory (EURL) has approved two methods for determining the presence of difructose anhydride III (DFA III) in the feed additive NITTEN DFAIII and in water.
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## Methods for DFA III Determination
### In the Feed Additive NITTEN DFAIII
The EURL recommends using High-Performance Liquid Chromatography (HPLC) with Refractive Index Detection (RID) to measure the purity and concentration of DFA III in the solid feed additive form. This method is known for its specificity, accuracy, and reproducibility.
### In Water (Diluted Solutions or Supplemented Water)
For liquid matrices such as drinking water or feed solutions, HPLC-RID or Ion Chromatography (IC) can be used to determine DFA III concentrations. The sample is prepared by appropriate dilution and filtration before analysis.
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## Additional Notes
The EURL emphasizes the use of validated, standardized analytical procedures with proper calibration using certified DFA III standards. Sample preparation steps and chromatographic conditions should ensure separation of DFA III from other saccharides or impurities.
These methods are intended to support enforcement and conformity assessment under EU feed additive regulations.
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NITTEN DFAIII, a white crystalline powder containing at least 99% of DFA III as the active substance, is intended for use in bovines. The recommended daily dosage for cows is 40 g/cow/day, while for other adult ruminants it is 1 g/10 kg BW (body weight) per day, and for other young ruminants it is 1.5 g/10 kg BW per day. For calves, the recommended daily dosage is 12 g/calf/day.
The authorization for DFA III (NITTEN DFAIII) is sought under Article 4(1) of Regulation (EC) No 1831/2003, categorized as a zootechnical additive and physiological condition stabilizer.
Upon the EURL's request, the Applicant verified the HPAEC-PAD method in a wheat bran meal-based matrix and reported acceptable performance characteristics. However, experimental data revealed significant interferences in the wheat bran-based matrices, leading to the EURL's recommendation of another analytical method (CEN/TS 15754) based on high performance anion exchange (HPAEC) coupled to pulse amperometric detection (PAD).
These recommendations will help ensure the quality and safety of NITTEN DFAIII in feed and water, supporting its effective use in bovine nutrition.
In the context of health-and-wellness and fitness-and-exercise, one might analyze the presence of DFA III in feed additive NITTEN DFAIII using data derived from High-Performance Liquid Chromatography (HPLC) with Refractive Index Detection (RID). In cases of diluted solutions or supplemented water, the data can also be obtained through HPLC-RID or Ion Chromatography (IC), following proper sample preparation.
